CHARACTERIZATION OF NOVEL CELL-LINES FROM PLEOMORPHIC ADENOMAS OF THEPAROTID-GLAND ESTABLISHED IN A COLLAGEN GEL SYSTEM

The pathobiology of salivary neoplasms can best be studied in a model system that reflects the native state of the tumor. The present study describes the use of a three-dimensional collagen gel (organoid) syste m in which pleomorphic adenomas of the parotid gland were propagated i n vitro. Five pleomorphic adenoma cultures were established as organoi d gels and compared with touch-preparations or cryopreserved specimens of native tumor. The organoid cultures demonstrated normal DNA conten t, the expression of myoepithelial cell proteins, and the production o f sulfated acid mucins; these cellular and secretory features mimicked those found in the archival specimens. Further, organoid cultures of pleomorphic adenoma could be initiated after monolayer culture, demons trating that culture on a plastic support does not alter the nature of the cells. Development of an in vitro culture system that maintains t he native state of pleomorphic adenoma is an important tool for studyi ng the pathobiology of these tumors.