Molecular and immunologic characterization of new isoforms of the Hevea brasiliensis latex allergen Hev b 7: Evidence of no cross-reactivity between Hev b 7 isoforms and potato patatin and proteins from avocado and banana
S. Sowka et al., Molecular and immunologic characterization of new isoforms of the Hevea brasiliensis latex allergen Hev b 7: Evidence of no cross-reactivity between Hev b 7 isoforms and potato patatin and proteins from avocado and banana, J ALLERG CL, 104(6), 1999, pp. 1302-1310
Background: Hev b 7 is a Hevea brasiliensis latex allergen with sequence id
entities of 39% to 42% to patatins recently identified as potato allergens.
The complementary DNAs encoding 2 different Hev b 7 isoforms were previous
ly reported.
Objective: The aim of this study was to determine the sequence variation of
Hev b 7 and to compare the IgE reactivity of individual isoforms in vitro
and in vivo. A further objective was to evaluate possible cross-reactivitie
s between Hev b 7 and patatins and proteins from banana and avocado.
Methods: An H brasiliensis lambda ZAP complementary DNA (cDNA) Library was
screened with use of a Hev b 7 cDNA probe. Four Hev b 7 isoforms Here produ
ced in recombinant form and their IgE-binding capacities a ere compared. Ig
E immunoblot inhibitions and ELISA inhibition assays were used to investiga
te the possible cross-reactivity between Hev b 7 and recombinant potato pat
atin and proteins from avocado and banana.
Results: Two new isoforms, S2 and D-2, were identified by sequencing 32 cDN
A clones with full-length coding regions. All 4 recombinant isoforms displa
yed esterase activity and identical IgE-binding capacities. The new isoform
s S2 and D-2 were evaluated in skin prick tests and provoked responses equi
valent to natural Hev b 7, No cross-reactivity was observed between Hev b 7
isoforms and potato patatin and proteins from avocado and banana.
Conclusions: All 4 recombinant Hev b 7 Isoforms have equivalent IgE-binding
capacity and therefore represent suitable reagents for the development of
in vitro and in vivo diagnostic tests. Hev b 7, patatins, and their homolog
s appear not to contribute to cross-reactivity in the Inter-fruit syndrome.