CONVERSION OF ALPHA-KETO ACIDS TO D-AMINO ACIDS BY COUPLING OF 4 ENZYME-REACTIONS

Authors
GALKIN A KULAKOVA L YAMAMOTO H TANIZAWA K TANAKA H ESAKI N SODA K
Citation
A. Galkin et al., CONVERSION OF ALPHA-KETO ACIDS TO D-AMINO ACIDS BY COUPLING OF 4 ENZYME-REACTIONS, Journal of fermentation and bioengineering, 83(3), 1997, pp. 299-300
Citations number
12
Categorie Soggetti
Food Science & Tenology","Biothechnology & Applied Migrobiology
Journal title
Journal of fermentation and bioengineering → ACNP
ISSN journal
0922338X
Volume
83
Issue
3
Year of publication
1997
Pages
299 - 300
Database
ISI
SICI code
0922-338X(1997)83:3<299:COAATD>2.0.ZU;2-D
Abstract
We developed a new procedure for stereospecific conversion of various alpha-keto acids to the corresponding D-amino acids with four thermost able enzymes: D-amino acid aminotransferase, alanine racemase, L-alani ne dehydrogenase and formate dehydrogenase. Optically pure D-enantiome rs of glutamate, phenylalanine and tyrosine were obtained with high co nversion rates.