K. Webb et al., Relationships among cell attachment, spreading, cytoskeletal organization,and migration rate for anchorage-dependent cells on model surfaces, J BIOMED MR, 49(3), 2000, pp. 362-368
Many research and commercial applications use a synthetic substrate which i
s seeded with cells in a serum-containing medium. The surface properties of
the material influence the composition of the adsorbed protein layer, whic
h subsequently regulates a variety of cell behaviors such as attachment, sp
reading, proliferation, migration, and differentiation. In this study, we e
xamined the relationships among cell attachment, spreading, cytoskeletal or
ganization, and migration rate for MC3T3-E1. osteoblasts on glass surfaces
modified with SOx, -NH2, -N+(CH3)(3), -SH, and -CH3 terminal silanes, We al
so studied the relationship between cell spread area and migration rate for
a variety of anchorage-dependent cell types on a model polymeric biomateri
al, poly(acrylonitrile-vinylchloride). Our results indicated that MC3T3-E1
osteoblast behavior was surface chemistry dependent, and varied with indivi
dual functional groups rather than general surface properties such as wetta
bility. In addition, cell migration rate was inversely related to cell spre
ad area for MC3T3-E1 osteoblasts on a variety of silane-modified surfaces a
s well as for different anchorage-dependent cell types on a model polymeric
biomaterial. Furthermore, the data revealed significant differences in mig
ration rate among different cell types on a common polymeric substrate, sug
gesting that cell type-specific differences must be considered when using,
selecting, or designing a substrate for research and therapeutic applicatio
ns. (C) 2000 John Wiley & Sons, Inc.