Characterization of a CSF-induced proliferating subpopulation of human peripheral blood monocytes by surface marker expression and cytokine production

Citation
M. Finnin et al., Characterization of a CSF-induced proliferating subpopulation of human peripheral blood monocytes by surface marker expression and cytokine production, J LEUK BIOL, 66(6), 1999, pp. 953-960
Citations number
53
Categorie Soggetti
Immunology
Journal title
JOURNAL OF LEUKOCYTE BIOLOGY
ISSN journal
07415400 → ACNP
Volume
66
Issue
6
Year of publication
1999
Pages
953 - 960
Database
ISI
SICI code
0741-5400(199912)66:6<953:COACPS>2.0.ZU;2-L
Abstract
The phenotype of a subpopulation(s) of human monocytes which has been shown to proliferate in vitro in response to macrophage colony-stimulating facto r (RI-CSF or CSF-1) and granulocyte-macrophage CSF (GM-CSF) is as yet unkno wn. To identify this proliferating subpopulation(s) rue demonstrated first that DNA synthesis was occurring under culture conditions suitable for flow cytometric evaluation. Flow cytometric analysis of surface antigen express ion identified that after 5 days of culture the proliferating subpopulation of monocytes expressed CD14, CD13, CD33, CD11b, CD11c, CD87, HLA-DR, CD45R O, and did not express CD86, CD34, CD80, CD4, CD16, and CD56, In addition, these proliferating monocytes (representing approximately 5% Of total monoc ytes) were shown to produce the proinflammatory cytokines interleukin-6 and tumor necrosis factor or in response to lipopolysaccharide stimulation. Fu rther characterization and subsequent isolation of this subpopulation of mo nocytes may provide new and important information necessary to understand i nflammatory diseases such as rheumatoid arthritis, where local proliferatio n at the site of inflammation may be a hey factor contributing to the chron icity of the disease.