M. Finnin et al., Characterization of a CSF-induced proliferating subpopulation of human peripheral blood monocytes by surface marker expression and cytokine production, J LEUK BIOL, 66(6), 1999, pp. 953-960
The phenotype of a subpopulation(s) of human monocytes which has been shown
to proliferate in vitro in response to macrophage colony-stimulating facto
r (RI-CSF or CSF-1) and granulocyte-macrophage CSF (GM-CSF) is as yet unkno
wn. To identify this proliferating subpopulation(s) rue demonstrated first
that DNA synthesis was occurring under culture conditions suitable for flow
cytometric evaluation. Flow cytometric analysis of surface antigen express
ion identified that after 5 days of culture the proliferating subpopulation
of monocytes expressed CD14, CD13, CD33, CD11b, CD11c, CD87, HLA-DR, CD45R
O, and did not express CD86, CD34, CD80, CD4, CD16, and CD56, In addition,
these proliferating monocytes (representing approximately 5% Of total monoc
ytes) were shown to produce the proinflammatory cytokines interleukin-6 and
tumor necrosis factor or in response to lipopolysaccharide stimulation. Fu
rther characterization and subsequent isolation of this subpopulation of mo
nocytes may provide new and important information necessary to understand i
nflammatory diseases such as rheumatoid arthritis, where local proliferatio
n at the site of inflammation may be a hey factor contributing to the chron
icity of the disease.