ALLOGENEIC-BLOOD STEM-CELL COLLECTION FOLLOWING MOBILIZATION WITH LOW-DOSE GRANULOCYTE-COLONY-STIMULATING FACTOR

Purpose: The optimal dose of granulocyte colony-stimulating factor (G- CSF) for mobilization of allegeneic-blood stem cells (AlloBSC) has yet to be determined. As part of a prospective trial, 41 related human le ukocyte antigen (HLA)-matched donors had blood cells mobilized with G- CSF at 5 mu g/kg/d by subcutaneous administration. The purpose of this trial was to monitor adverse effects during G-CSF administration and stem-cell collection, to determine the optimal timing for stem-cell co llection, and to determine the cellular composition of stem-cell produ cts following G-CSF administration. Patients and Methods: The median d onor age was 42 years. Apheresis began on day 4 of G-CSF administratio n. At least three daily 12-L apheresis collections were performed on e ach donor. A minimum of 1.0 x 10(6) CD34(+) cells/kg (recipient weight ) and 8.0 x 10(8) mononuclear cells/kg were collected from each donor. All collections were cryopreserved in 5% dimethyl sulfoxide and 6% hy droxyethyl starch. Results: toxicities associated with G-CSF administr ation and the apheresis process included myalgias/arthralgias (83%), h eadache (44%), fever (27%), and chills (22%). The median baseline plat elet count of 242 x 10(6)/mL decreased to 221, 155, and 119 x 10(6)/mL on days 4, 5, and 6 of G-CSF administration, respectively. Median num bers of CD34(+) cells in collections 1, 2, and 3 were 1.99, 2.52, and 3.13 x 10(6)/kg, respectively. The percentage and total number of CD4( +), CD8(+), and CD56(+)/CD3(-) cells remained relatively constant duri ng the three collections. Median total numbers of cells were as follow s: CD34(+), 7.73 x 10(6)/kg; and lymphocytes, 6.93 x 10(8)/kg. Conclus ion: Relatively low doses of G-CSF can mobilize sufficient numbers of AlloBSC safely and efficiently. (C) 1997 by American Society of Clinic al Oncology.