Morphological and morphometric analysis of human detrusor mitochondria with urodynamic correlation after partial bladder outlet obstruction

Purpose: We correlated ultrastructural changes in mitochondria in the human detrusor with the severity of partial bladder outlet obstruction on urodyn amics, Materials and Methods: We recruited into the study 52 men with and without bladder outlet obstruction symptoms. The severity of partial bladder outlet obstruction was determined by pressure flow study. Random detrusor biopsy specimens obtained by cystoscopy were fixed immediately and processed for t ransmission electron microscopic observation. Random areas were photographe d for further morphological and morphometric analysis using mitochondrial d amage score and stereological principles. Results: Mitochondrial damage score and mean mitochondrial volume strongly correlated with the urodynamic severity of partial bladder outlet obstructi on, while mitochondrial volume density, surface density of the mitochondria l outer membrane and number of mitochondria per unit of cytoplasm area did not significantly correlate with severity. Conclusions: Detrusor mitochondrial swelling and structural destruction inc reased with the severity of partial bladder outlet obstruction. These chang es may be associated with impaired mitochondrial function and oxidative met abolism after partial bladder outlet obstruction. Detrusor mitochondrial da mage may explain voiding dysfunction after partial bladder outlet obstructi on develops.