Mouse cDNA sequences encoding alpha, beta, and gamma adducins were cloned f
rom a mouse reticulocyte cDNA library. The purified clones contain alternat
ively spliced exons from all three adducin genes. In the case of alpha and
beta, the inclusion of che alternatively spliced exons results in truncated
polypeptide isoforms (called alpha-2 and beta-2). The mouse predicted amin
o acid sequences are compared with published rat and human sequences. For c
ompletion of this comparison. cDNA encoding the rat beta-1 carboxy terminus
was cloned by PCR. The carboxy terminal region containing MARCKS homology,
calmodulin-binding region-2, and spectrin-actin-binding site, is conserved
among alpha-1, beta-1, and gamma-1 isoforms in mouse, rat, and humans. We
also report here the localization of the gene encoding gamma adducin (Add3)
to murine Chr 19, in a region that shows conserved synteny with human Chr
10.