RAPID ISOLATION OF YEAST PLASMIDS AS NATIVE CHROMATIN

Many regions of chromatin are subject to dynamic changes. We have deve loped a rapid method for isolation of small chromatin templates from y east which will facilitate biochemical analysis of chromatin compositi on. Using the PHO5 promoter we show that templates prepared from cells grown in inducing or repressing conditions show native chromatin stru ctures. This method may be widely applicable as the chromatin structur es at a centromere, at ARS1 and at part of the lacZ region on two othe r plasmids are preserved after chromatin isolation.