Identification of mango cultivars of Thailand and evaluation of their genetic variation using the amplified fragments by simple sequence repeat-(SSR-) anchored primers

Twenty-two mango cultivars (Mangifera indica Linn,), including 13 Thai, fou r Florida, three Indian, one Indonesian, and one Philippine cultivar, were examined for 40 simple sequence repeat (SSR-) anchored primers of 15-18 oli gonucleotides which were provided by Biotechnology Laboratory, University o f British Columbia. Of the 40 primers screened, seven primers gave reproduc ible, polymorphic DNA amplification patterns, and were selected to construc t a DNA fingerprinting table to distinguish the genotypes of mango. The num ber of bands generated ranged from 8 to 21 per primer. By banding patterns obtained from these seven primers, each cultivar in this study could be dis tinguished from the others, indicating that PCR by using SSR-anchored prime rs was an efficient method for cultivar identification. Similarity coeffici ents were calculated based on 56 selected bands and UPGMA clustering analys is was performed. Two Thai mango cultivars ('Nang Klangwan' and 'Nong Saeng ') were found to be very far distant of the genetic relationship from the o ther cultivars. The remaining 11 Thai cultivars were classified into three groups based on the dendrogram. Seven cultivars were placed in the same gro up as two Florida cultivar ('Brooks' and 'Edward'), one Philippine cultivar ('Carabao') and one Indonesian cultivar ('Arumanis'). Four other Thai cult ivars were scattered into two groups; each group contained Indian cultivars . Our analysis did not give clearly distinction between the polyembryonic a nd monoembryonic seed races. (C) 1999 Elsevier Science B.V. All rights rese rved.