Cytomegalovirus-mediated modulation of adhesion molecule expression by human arterial and microvascular endothelial cells

Background Cytomegalovirus (CMV), a betaherpes-virus associated with allogr aft rejection, infects the endothelium, the cellular interface between allo graft tissue and the host immune system. Because of recent appreciation of the phenotypic diversity of endothelial cells (EC) from different vascular compartments, controversy now exists on the universality of CMV-mediated ad hesion molecule induction previously described on umbilical vein EC. Theref ore, we herein extend these previous studies to arterial and microvascular EC, which represent sites of vascular rejection. Methods. Human coronary artery, aortic, umbilical artery, and microvascular EC were mock or CMV-infected and/or treated with tumor necrosis factor-alp ha before flow cytometric and immunohistochemical analysis. Results. CMV directly enhanced intercellular adhesion molecule-1 on all EC isolates but did not induce E-selectin or vascular cell adhesion molecule-1 . Furthermore, CMV-infected EC were refractory to tumor necrosis factor-alp ha-mediated induction of these molecules. Conclusion. CMV-induced modulations of adhesion molecule expression, which may affect allograft immunogenicity, seem common to all EC regardless of va scular origin.