This investigation demonstrates that in situ respirometry can be an effecti
ve tool to manage the removal of an inhibitory substrate in a sequencing ba
tch reactor (SBR). Data collected enabled the determination of an optimum o
perating cycle for the effective treatment of a synthetic wastewater contai
ning up to 1300 mg/l phenol as the sole carbon source.
Oxygen uptake rates were monitored in situ at various stages of a bench-sca
le sequencing batch reactor. Respirometry was used as an indicator of micro
bial activity and substrate utilisation. Although the profile of the Oxygen
Uptake Rate (OUR) followed closely that of phenol substrate removal, any c
orrelation between the OUR and soluble residual substrate COD was specific
to that system. A high OUR Value corresponded to the feed period; at the en
d of the cycle, when the substrate was depleted, the OUR Value was low. A p
lot of OUR values provided a good indication of the biological activity in
the reactior.
It was possible to select an optimum operating cycle using the oxygen uptak
e rates as an indicator for the removal of phenol. The initial operating cy
cle was 24 h, which included phases for filling, reaction, quiescence and d
ecanting. From oxygen uptake rates and corresponding soluble COD values of
the remaining substrate, data showed that the 4 h operating cycle was able
to achieve similar substrate removal efficiencies. At a SBR loading rate of
3.12 kg phenol m(-3)d(-1), removal of 97% COD was achieved at the end of t
he cycle. The reactor was operated at a SRT of 4-27 days with biomass conce
ntrations ranging from 3500-3900 mg/l. (C) 1999 Elsevier Science Ltd. All r
ights reserved.