D. Tadi et al., Selection of genes repressed by cAMP that are induced by nutritional limitation in Saccharomyces cerevisiae, YEAST, 15(16), 1999, pp. 1733-1745
DNA-lacZ fusion libraries of yeast Saccharomyces cerevisiae were used to se
lect genes coordinately regulated by the Ras-cAMP-cAPK signalling pathway.
Sixteen new genes (AGP1, APE2, APE3, FPS1, GUT2, MDH2, PLB2, PYK2, RNR3, SU
R1, UGA1, YHR033w, PBR006w, YHR143w, YMR086w and YOR173w) were found to be
repressed by cAMP. Most of these genes encode for metabolic enzymes and are
induced by nutritional limitations. These common properties suggest a role
of this pathway in the metabolic adjustment of the cell to nutritional var
iations. The induction of 10 of these genes is reduced in the msn2,msn4 dou
ble mutant, which emphasizes the role of the Msn2/4p transcriptional activa
tors in mediating the Ras-cAMP-cAPK signalling pathway. The Msn2p/ Msn4p-in
dependent expression of the six other genes suggests the existence of other
regulatory systems under the control of this pathway. Copyright (C) 1999 J
ohn Wiley & Sons, Ltd.