Systematic analysis of S-cerevisiae chromosome VIII genes

Citation
R. Niedenthal et al., Systematic analysis of S-cerevisiae chromosome VIII genes, YEAST, 15(16), 1999, pp. 1775-1796
Citations number
34
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
YEAST
ISSN journal
0749503X → ACNP
Volume
15
Issue
16
Year of publication
1999
Pages
1775 - 1796
Database
ISI
SICI code
0749-503X(199912)15:16<1775:SAOSCV>2.0.ZU;2-U
Abstract
To begin genome-wide functional analysis, we analysed the consequences of d eleting each of the 265 genes of chromosome VIII of Saccharomyces cerevisia e. For 33% of the deletion strains a growth phenotype could be detected: 18 % of the genes are essential for growth on complete glucose medium, and 15% grow significantly more slowly than the wild-type strain or exhibit a cond itional phenotype when incubated under one of 20 different growth condition s. Two-thirds of the mutants that exhibit conditional phenotypes are pleiot ropic; about one-third of the mutants exhibit only one phenotype. We also m easured the level of expression directed by the promoter of each gene. Abou t half of the promoters direct detectable transcription in rich glucose med ium, and most of these exhibited only low or medium activity. Only 1% of th e genes are expressed at about the same level as ACT1. The number of active promoters increased to 76% upon growth on a non-fermentable carbon source, and to 93% in minimal glucose medium. The majority of promoters fluctuated in strength, depending on the medium. Copyright (C) 1999 John Wiley & Sons , Ltd.