Analysis of human gene expression using high-density cDNA filters

Most technical strategies for the analysis of gene expression in tissues ar e able to study only one protein or RNA product at the same time. A new rec ent method referred to as << DNA array >> or a high density filter a is abl e to analyze simultaneously several hundreds of different genes. The DNA ar ray is a nylon membrane on which are spotted equal amounts of cDNAs corresp onding to different genes. This filter is hybridized with a << complex >> p robe synthesized with mRNA derived from the tissue analyzed The result give s a global profile of gene expression within the tissue and allows quantita tive and comparative analysis between different tissues or cell types.