L. Vigilant, An evaluation of techniques for the extraction and amplification of DNA from naturally shed hairs, BIOL CHEM, 380(11), 1999, pp. 1329-1331
Hair can be a valuable source of DNA for the noninvasive study of human and
nonhuman populations. However hairs contain extremely small quantities of
DNA, making the method used to extract the DNA of paramount importance. Thi
s study compares the effectiveness of 4 different methods of DNA extraction
from shed chimpanzee hair, as measured by the ability to amplify mtDNA tar
gets using PCR. The most successful method is also the simplest, requiring
only digestion of the root end in a buffer compatible with subsequent PCR w
ithout a prior purification or extraction step. Strategies to non-specifica
lly preamplify the template are not successful with DNA from stored shed ha
irs.