Platelet derived growth factor releasing chitosan sponge for periodontal bone regeneration

With an aim of improving bone regeneration, chitosan sponge containing plat elet-derived growth factor-BE (PDGF-BB) were developed. For fabrication of chitosan sponge, chitosan solution was freeze-dried, crosslinked and freeze -dried again. PDGF-BB was incorporated into the chitosan sponge by soaking chitosan sponge into the PDGF-BE solution. Release kinetics of PDGF-BB, cel l attachment, proliferation capacity and bony regenerative potentials of PD GF-BB-loaded chitosan sponge were investigated. Prepared chitosan sponge re tained porous structure with 100 mu m pore diameter that was suitable for c ellular migration and growth. Release rate of PDGF-BB could be controlled b y varying initial loading content of PDGF-EB to obtain optimal therapeutic efficacy. PDGF-BB-loaded chitosan sponge induced significantly high cell at tachment and proliferation level, which indicated good cellular adaptabilit y. PDGF-BB-loaded chitosan sponge demonstrated marked increase in new bone formation and rapid calcification. Degradation of the chitosan sponge was p roceeded at defect site and subsequently replaced with new bone. Histomorph ometric analysis confirmed that PDGF-BB-loaded chitosan sponge significantl y induced new bone formation. These results suggested that chitosan sponge and PDGF-BB-loaded chitosan sponge may be beneficial to enhance periodontal bone regeneration. (C) 1999 Elsevier Science Ltd. All rights reserved.