Background: Research about parity or breastfeeding experience and its relat
ionship with milk production in humans is limited. The purpose of this stud
y was to determine if any difference in milk volume occurred among mothers
with and without breastfeeding experience who used either a single or doubl
e breast pumping regimen the first 5 weeks postpartum. Methods: A convenien
ce sample of 39 mothers of nonnursing preterm infants participated from two
tertiary care centers in the midwestern United States. The sequential sing
le pumping group consisted of 20 mothers, 7 of whom had previous breastfeed
ing experience; the simultaneous double pumping group consisted of 19 mothe
rs, 2 of whom had previous breastfeeding experience. Income and pumping gro
up regimen were used as blocking variables, and average frequency of kangar
oo care per week and average frequency of breast pumping per week were cova
riants in the repented measures analysis of variance. Results: Mothers with
previous breastfeeding experience had greater milk weights over time, but
weights were nor significantly different when compared with those mothers w
ith no previous breastfeeding experience. Additional analysis with the cova
riants of pumping frequency and kangaroo care, and with the independent var
iables of group, breastfeeding experience, and income resulted in statistic
ally significantly greater milk yield in the women with previous breastfeed
ing experience. Conclusions: The findings of the two repeated analyses indi
cated the complexity of the milk production response, and the importance of
considerations of environmental as well as physiologic factors.