Modifying effect of heparin sulfate from human blood leukemic cells on themitogenic activity of fibroblast growth factor and [C-14]-arachidonic acidmetabolism in culture of stromal fibroblasts of rat bone marrow

Modifying effect of heparan sulfate (HS) from human blood leukemic cells on the mitogenic activity of basic fibroblast growth factor (bFGF) has been i nvestigated in culture of stromal fibroblasts of rat bone marrow. bFGF stim ulated growth of precursor cells and enhanced significantly the biosynthesi s of prostaglandin (PG)E-2 from incorporated [C-14]-arachidonic acid (AA) i n fibroblasts. Antiproliferative effect of HS on colony formation of fibrob lasts (78% relative to control) correlated with an essential decrease of PG E(2) production in cells. Similar growth inhibition was revealed in culture with cyclooxygenase inhibitor indomethacin. Both HS and indomethacin aboli shed completely the mitogenic effect of bFGF. Results demonstrate a close i nterelationship of HS and bFGF action with cyclooxygenase path of AA metabo lism.