Forssman pentasaccharide and polyvalent Ga1 beta 1 -> 4GlcNAc as major ligands with affinity for Caragana arborescens agglutinin

Citation
Am. Wu et al., Forssman pentasaccharide and polyvalent Ga1 beta 1 -> 4GlcNAc as major ligands with affinity for Caragana arborescens agglutinin, FEBS LETTER, 463(3), 1999, pp. 225-230
Citations number
29
Categorie Soggetti
Biochemistry & Biophysics
Journal title
FEBS LETTERS
ISSN journal
00145793 → ACNP
Volume
463
Issue
3
Year of publication
1999
Pages
225 - 230
Database
ISI
SICI code
0014-5793(199912)463:3<225:FPAPGB>2.0.ZU;2-V
Abstract
The binding properties of Caragana arborescens agglutinin (CAA, pea tree ag glutinin) were studied by enzyme linked lectinosorbent assay (ELLSA) and by inhibition of CAA-glycan interaction. Among glycoproteins (gps) tested, CA A reacted strongly with asialo bird nest gp, asialo rat sublingual gp, huma n Tamm-Horsfall Sd(a(+)) urinary gp (THGP) and asialo THGP that are rich in GalNAc alpha 1-->, GalNAc beta 1--> and/or Gal beta 1 --> 4GlcNAc residues . CAA also bound tightly with multivalent Gal beta 1 --> 4GlcNAc (mII) cont aining glycoproteins (human blood group precursor gps, asialo fetuin) and a sialo ovine salivary glycoprotein (Tn, GalNAc alpha 1 --> Ser/Thr) but CAA reacted poorly of not at all with sialylated glycoproteins tested, Of the s ugars tested for inhibition of binding, Forssman pentasaccharide (F-p, GalN Ac alpha 1 --> 3GalNAc beta 1 --> 3Gal alpha 1 --> 4Gal beta 1 --> 4Glc) wa s the best, It nas about 2.3, 9.5 and 52.6 times more active than Gal beta 1 --> 4GlcNAc, GalNAc and Gal, respectively, and about 1.9 times more activ e than tri-antennary Gal beta 1 --> 4GlcNAc (Tri-II), These results suggest that this agglutinin is mainly specific for F-p, mil and Tn clusters. This property can be used to detect human abnormal glycotopes related to F-p an d unmasked mII/Tn clusters and to study cell growth and differentiation giv en the lack of toxicity of this lectin toward mouse fibroblast cells, (C) 1 999 Federation of European Biochemical Societies.