Prevalence of germline truncating mutations in ATM in women with a second breast cancer after radiation therapy for a contralateral tumor

Patients treated with conservative surgery and radiation therapy for early- stage breast cancer develop a contralateral breast cancer at a rate of appr oximately 0.75% per year. Ataxia-telangiectasia (AT) is an autosomal recess ive disease that is characterized by increased sensitivity to ionizing radi ation (IR) and cancer susceptibility. Epidemiologic studies have suggested that AT carriers, who comprise 1% of the population, may be at an increased risk for developing breast cancer, particularly after exposure to IR. To t est this hypothesis, we analyzed blood samples from 57 patients who develop ed a contralateral breast cancer at least 6 months after completion of radi ation therapy for an initial breast tumor. A cDNA-based truncation assay in yeast: was used to test for heterozygous mutations in the ATM gene, which is responsible for AT. No mutations were detected. Our findings fail to sup port the hypothesis that AT carriers account for a significant fraction of breast cancer cases arising in women after exposure to radiation. Genes Chr omosomes Cancer 27:124-129, 2000. (C) 2000 Wiley-Liss, Inc.