Identification of common bean alleles resistant to anthracnose using RAPD

RAPD markers were identified close to common bean alleles responsible for r esistance to the fungus Colletotrichum lindemuthianum and may be useful in selecting plants resistant to this pathogen, DNA from F-2 plants of the cro sses Carioca 300V x P45, Carioca 300V x Ouro and P24 x Ouro was amplified b y RAPD. Line P45 has the Co.4 allele for resistance, and the Ouro cultivar has the Co.5 allele. The primer OPC08 amplified a DNA fragment of about 105 9 bp linked to the Co.4 allele, The recombination frequency was 0.133 (SE = 0.039; 95% CI = 0.056-0.211). Using the primer OPF10 a DNA fragment of abo ut 912 bp was amplified and found to be associated with the Co.5 allele. Th e recombination frequency was 0.115 (SE = 0.038; 95% CI = 0.041-0.189). A s econd marker (1122 pb) amplified by the OPR03 primer was identified in the population P24 x Ouro. The recombination frequency for this marker was 0.36 3 (SE = 0.081; 95% CI = 0.205-0.522). Both these markers flanked the Co.5 a llele. The markers identified in this study may be useful in identifying li nes with the Co.4 and Co.5 alleles.