URINARY AND BILIARY METABOLITES OF PYRENE IN RAINBOW-TROUT (ONCORHYNCHUS-MYKISS)

1. Pyrene was administered i.p. as a single dose to trout (Oncorhynchu s mykiss). Urine was collected continuously for 3 days and bile sample d at the end of this period. Pyrene metabolites in these biological fl uids were identified by 1H-nmr spectrometry, glc-ms and hplc-ms. 2. 1- Hydroxypyrene was the major oxidation metabolite in the urine and bile . Small amounts of 1,6-dihydroxypyrene and a putative 1,8-dihydroxypyr ene metabolite also were detected. Unchanged pyrene was not found in a ny of these biological fluids. 3. Both free and conjugated metabolites of pyrene were found in the bile and urine. The majority of the pyren e metabolites in the bile were conjugated with glucuronic acid or sulp hate.