Evaluation of sandwich ELISA galactomannan test in samples of positive LA test and positive aspergillus antibody

Objective The detection of circulating Aspergillus galactomannan antigen is a useful tool for serodiagnosis of aspergillosis, However, the latex agglu tination test for the detection of galactomannan is not completely reliable due to it's low sensitivity. The sandwich ELISA was developed to achieve h igh sensitivity. Materials The sandwich immunocapture ELISA was evaluated b y testing 56 sero-positive and 56 sero-negative samples of circulating gala ctomannan detected by LA test retrospectively. Results Sixty of the samples were positive for galactomannan as measured by sandwich ELISA, Fifteen sam ples out of 56 samples negative by LA test were positive by ELISA and 4 sam ples out of 56 samples positive by LA test were negative by ELISA. Among 47 serum samples positive for anti-Aspergillus antibody, 14 samples were posi tive by ELISA, Conclusion In conclusion, galactomannan may be detected in m ore samples of by the new sandwich ELISA than by LA test.