Feedlot cattle normally retain less than 20% of their dietary nitrogen inta
ke. Sixty to 80% of the nitrogen excreted is normally lost through volatili
zation of ammonia, which is primarily generated from urea. This loss of amm
onia nitrogen pollutes the environment and creates an unfavorable ratio of
nitrogen to phosphorous (N:P) in the waste for crop growth. Two urease inhi
bitors, cyclohexylphosphoric triamide (CHPT) and N-(n-butyl) thiophosphoric
triamide (NBPT) were evaluated for their ability to reduce the rate of ure
a hydrolysis in beef cattle feedlot pens. Initially, a total of six pens we
re used, two pens per treatment, with approximately 70 cattle per pen, and
a single topical application of CHPT or NBPT at 20 mg/kg of manure. Essenti
ally no urea was found in untreated pens. However, with CHPT treatment, 2 g
of urea/kg of dry manure accumulated by d 4, and all gradually disappeared
by d 11; NBPT conserved 3 and 3.5 g of urea/kg by d 4 and 9, respectively,
and it had disappeared by d 14 (treatment [trt] x day, P = .003), A second
study involved application of NBPT weekly for 6 wk. This caused urea to ac
cumulate to a peak concentration of 17 g/kg of manure by d 30 (trt x day(2)
, P = .001). Once the treatment was stopped the urea concentration began to
decrease. When the NBPT was applied weekly, the concentration of ammonia i
n the waste was less for the treated pens (trt x day, P = .01), the total n
itrogen was greater (trt x day, P = .04), pH tended to be lower (trt x day,
P = .10), and the total volatile acids were not different (trt x day, P =
.51) from untreated pens. We concluded that urease inhibitors could be used
to control ammonia emissions from animal wastes, prevent environmental dam
age, and produce a more balanced (N:P) fertilizer from manure.