J. Mao et al., Reproductive, metabolic, and endocrine responses to feed restriction and GnRH treatment in primiparous, lactating sows, J ANIM SCI, 77(3), 1999, pp. 724-735
The current experiment was carried out to determine whether exogenous GnRH
treatment in primiparous, lactating sows undergoing feed restriction would
improve reproductive performance after weaning. Sows were allocated to one
of three treatments: AA sows (n = 8) were fed to appetite throughout a 28-d
lactation, AR (n = 12) and AR + GnRH (n = 12) sows were fed as AA sows fro
m farrowing to d 21 of lactation, and feed intake was reduced to 50% of the
ad libitum intakes from d 22 to 28. The AR + GnRH sows received 800 ng of
GnRH i.v. every 6 h from d 22 to 28 of lactation, and AA. and AR sows recei
ved saline. Sow weight, backfat, and litter weight were recorded weekly. Wi
thin 2 d after farrowing, litter size was standardized to 8 to 10. At d 17
of lactation, an indwelling jugular catheter was surgically implanted in ea
ch sow,,Blood samples were taken for characterization of plasma LH, FSH, in
sulin, IGF-I, and leptin by RIA at d 21 and before and after weaning on d 2
8 of lactation. After weaning, all sows were given ad libitum access to fee
d, checked for onset of standing estrus twice daily with mature vasectomize
d boars, and inseminated 12 and 24 h after onset of standing estrus with po
oled semen from the same fertile boars (3 x 10(9) sperm/AI). After breeding
, feed allowance was reduced to NRC (1988) requirements for gestation. At d
28 +/- 3 of gestation, sows were killed and ovulation rate and embryo surv
ival were determined. Restricted sows lost more weight during lactation tha
n AA sows (P < .02). During the period of feed restriction, plasma IGF-I an
d postprandial insulin and leptin in AR and AR + GnRH sows, and LH pulse fr
equency in AR sows, were lower than those in AA sows (P < .04). Association
s (P < .004) between plasma insulin and leptin and between leptin and mean
LH concentrations were established. The LH pulse frequency in AR + GnRH sow
s did not differ from that in AA sows before weaning. After weaning, maximu
m, mean, and minimum LH concentrations in the AA and AR sows, and FSH conce
ntrations in AR sows, increased (P < .05) in response to weaning. Paradoxic
ally, GnRH treatment in lactation seemed to suppress the expected LH and FS
H responses to weaning. Ovulation rate and embryo survival were not differe
nt among the three groups. In conclusion, although exogenous GnRH therapy r
estored LH secretion in feed-restricted sows, it did not improve overall re
productive performance.