Theoretical investigation of fructose 1,6-diphosphate production and simultaneous ATP regeneration by conjugated enzymes in an ultrafiltration hollow-fiber reactor

The performance of an ultrafiltration hollow-fiber reactor, in which enzyma tic synthesis of fructose 1,6-diphosphate (FDP) from glucose and enzymatic ATP regeneration are performed simultaneously, was analyzed theoretically. The reaction system consists of three-step synthetic reactions catalyzed by glucokinase (GB), phosphoglucose isomerase and phosphofructokinase, and th e ATP regeneration reaction catalyzed by acetate kinase. Based on a simple analytical model developed previously in which the liquid flowing in a tube was assumed to be plug flow and the radial concentration gradients in the tubes and shell side space were both neglected, a computer program was deve loped to calculate the concentration profiles of all the components along t he Row direction in the tubes and shell side space of the reactor. From the FDP concentrations at the reactor outlet calculated under various operatio nal conditions, reactor performances such as the FDP yield and the ATP recy cle number were determined. The calculation showed the interesting phenomen on that under some conditions the FDP yield was higher when GK concentratio n was lower.