Experimental investigation of fructose 1,6-diphosphate production and simultaneous ATP regeneration by conjugated enzymes in am ultrafiltration hollow-fiber reactor

The enzymatic synthesis of fructose 1,6-diphosphate (FDF) from glucose and the enzymatic ATP regeneration were performed simultaneously in an ultrafil tration hollow-fiber reactor using both the purified enzymes and the crude cell extract of Bacillus stearothermophilus. The process consisted of the t hree-step synthetic reactions catalyzed by glucokinase, phosphoglucose isom erase and phosphofructokinase, and the ATP regeneration reaction catalyzed by acetate kinase. The experimental results of the yield and the recycle nu mber agreed well with the theoretical predictions calculated using a comput er program developed in our preceding study. This proved the validity of th is computer program for predicting or analyzing the reactor performance.