Formulation of defined media for carbon monoxide fermentation by Eubacterium limosum KIST612 and the growth characteristics of the bacterium

Phosphate-buffered (PBBM) and carbonate-buffered (CBBM) basal media were us ed in the formulation of defined media for the cultivation of Eubacterium l imosum KIST612 with carbon monoxide (CO) as the sole energy source. The bac terium was adapted to the minimal media by sequential passage in media cont aining casamino acids and those containing ammonium chloride in the place o f yeast extract. Biological growth was slower with a lower growth yield in the defined minimal media than in PBBM or CBBM. More butyrate was produced in phosphate-buffered media than in carbonate-buffered media. The bacteria grew without any organic nitrogen in the presence of trace quantities of bi otin and pantothenic acid, Anaerobic digester fluid stimulated bacterial gr owth.