Quantification of the xenoestrogens 4-tert.-octylphenol and bisphenol A inwater and in fish tissue based on microwave assisted extraction, solid-phase extraction and liquid chromatography-mass spectrometry
Sn. Pedersen et C. Lindholst, Quantification of the xenoestrogens 4-tert.-octylphenol and bisphenol A inwater and in fish tissue based on microwave assisted extraction, solid-phase extraction and liquid chromatography-mass spectrometry, J CHROMAT A, 864(1), 1999, pp. 17-24
Extraction methods were developed for quantification of the xenoestrogens 4
-tert.-octylphenol (tOP) and bisphenol A (BPA) in water and in liver and mu
scle tissue from the rainbow trout (Oncorhynchus mykiss). The extraction of
tOP and BPA from tissue samples was carried out using microwave-assisted s
olvent extraction (MASE) followed by solid-phase extraction (SPE). Water sa
mples were extracted using only SPE. For the quantification of tOP and BPA,
liquid chromatography mass spectrometry (LC-MS) equipped with an atmospher
ic pressure chemical ionisation interface (APCI) was applied. The combined
methods for tissue extraction allow the use of small sample amounts of live
r or muscle (typically 1 g), low volumes of solvent (20 mi), and short extr
action times (25 min). Limits of quantification of tOP in tissue samples we
re found to be approximately 10 ng/g in muscle and 50 ng/g in liver (both b
ased on 1 g of fresh tissue). The corresponding values for BPA were approxi
mately 50 ng/g in both muscle and liver tissue. In water, the limit of quan
tification for tOP and BPA was approximately 0.1 mu g/l (based on 100 mi sa
mple size). (C) 1999 Elsevier Science BN. All rights reserved.