Constitutive expression of the 25-kDa heat shock protein Hsp25 reveals novel parasagittal bands of Purkinje cells in the adult mouse cerebellar cortex

Citation
Cl. Armstrong et al., Constitutive expression of the 25-kDa heat shock protein Hsp25 reveals novel parasagittal bands of Purkinje cells in the adult mouse cerebellar cortex, J COMP NEUR, 416(3), 2000, pp. 383-397
Citations number
69
Categorie Soggetti
Neurosciences & Behavoir
Journal title
JOURNAL OF COMPARATIVE NEUROLOGY
ISSN journal
00219967 → ACNP
Volume
416
Issue
3
Year of publication
2000
Pages
383 - 397
Database
ISI
SICI code
0021-9967(20000117)416:3<383:CEOT2H>2.0.ZU;2-C
Abstract
Despite the reported absence of the 25-kDa heat shock protein Hsp25 in the rodent cerebellum, we have determined that Hsp25 is constitutively expresse d in a subset of Purkinje cells in the adult cerebellum of the mouse. Mo ot her cerebellar neurons are Hsp25 immunoreactive, but there is weak staining associated with blood vessels. In the vermis, Hsp25-immunoreactive Purkinj e cells are confined to two regions: one in lobules VI/VII, the other in lo bules IX/X. In each region, only a subset of the Purkinje cells is immunore active. These cells are grouped in five parasagittal bands arranged symmetr ically about the midline. The boundaries of these expression domains corres pond to transverse zones previously inferred from other expression patterns . A third Hsp25-immunopositive domain is seen in the paraflocculus and floc culus. Again, only a subset of Purkinje cells within the paraflocculus and flocculus express Hsp25, revealing three distinct bands. Previous descripti ons of compartmentation antigens have not differentiated between adult popu lations of Purkinje cells in these regions, suggesting that Hsp25 is a nove l compartmentation antigen in the adult cerebellum. J. Comp. Neurol. 416:38 3-397, 2000. 2000 (C) Wiley-Liss, Inc.