GAS-CHROMATOGRAPHIC MASS-SPECTROMETRY AND GAS-CHROMATOGRAPHIC FOURIER-TRANSFORM INFRARED-SPECTROSCOPY ASSAY FOR THE SIMULTANEOUS IDENTIFICATION OF FENTANYL METABOLITES

Authors
GUITTON J DESAGE M ALAMERCERY S DUTRUCH L DAUTRAIX S PERDRIX JP BRAZIER JL
Citation
J. Guitton et al., GAS-CHROMATOGRAPHIC MASS-SPECTROMETRY AND GAS-CHROMATOGRAPHIC FOURIER-TRANSFORM INFRARED-SPECTROSCOPY ASSAY FOR THE SIMULTANEOUS IDENTIFICATION OF FENTANYL METABOLITES, Journal of chromatography B. Biomedical sciences and applications, 693(1), 1997, pp. 59-70
Citations number
14
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications → ACNP
ISSN journal
13872273
Volume
693
Issue
1
Year of publication
1997
Pages
59 - 70
Database
ISI
SICI code
0378-4347(1997)693:1<59:GMAGF>2.0.ZU;2-F
Abstract
Fentanyl, a synthetic opioid, undergoes important biotransformation to several metabolites. A gas chromatographic-mass spectrometric assay w as applied for the simultaneous analysis of fentanyl and its major met abolites in biological samples. The identification of different metabo lites was performed by gas chromatography-mass spectrometry (electroni c impact and chemical ionisation modes) and gas chromatography-Fourier transform infrared spectroscopy. In the present study, rat and human microsomes incubation mixtures and human urines were analysed. In vitr o formation of already known fentanyl metabolites was confirmed. The p resence of metabolites not previously detected in human urine is descr ibed.