QUANTITATIVE-DETERMINATION OF PAROXETINE IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND ULTRAVIOLET DETECTION

An accurate, reliable procedure was developed for kinetic and therapeu tic monitoring of paroxetine in human plasma. Steady-state plasma leve ls of paroxetine were measured for 18 geriatric patients (mean age 75) in a double-blinded study. Paroxetine doses ranged from 10 to 40 mg/d ay. The assay was suitable for patients on concurrent medications, and a small sample volume (1 ml) of patient plasma was used with sufficie nt sensitivity and specificity. After extraction and separation on a B eckman, Ultrasphere 5-mu m C-18 column (150X2 mm I.D.), the recovery ( mean+S.D.) for paroxetine was determined to be 86.5+/-5.2%. The limit of quantitation for paroxetine in this assay was 5 ng/ml. Inter-assay reproducibility (C.V.) for the patient samples and quality controls ra nged from 3.7 to 7.6%.