HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF PEPTIDE-T IN RABBIT PLASMA WITH ONLINE COLUMN ENRICHMENT

The present paper describes the development of a simple and sensitive analytical method for quantification of Peptide T (PT) in rabbit plasm a, using standard analytical equipment and on-line column enrichment, without prior extraction, clean-up or derivatization. The analytical p rocedure was found to be accurate, precise and linear. The accuracy wa s 100% (range 97-103%) and the mean precision was 8% (range 3-14%) for all (n=6) concentrations (0, 15, 50, 100 and 200 ng/ml). The total re covery was found to be approximately 80%, and it was found to be depen dent upon the injection rate onto the extraction column. The correlati on between added and found concentrations was 0.9982, and the limit of detection was estimated to be around 5 ng/ml. The method is therefore found to be suitable for bioavailability studies, involving Peptide T , in rabbits.