SIMPLE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF ACYCLOVIR IN HUMAN PLASMA USING FLUORESCENCE DETECTION

A simple high-performance liquid chromatographic method using fluoresc ence detection was developed for the determination of acyclovir in hum an plasma. The method entailed direct injection of the plasma sample a fter deproteination. It is both specific and sensitive with a detectio n limit of 30 ng/ml at a signal-to-noise ratio of 3:1, and is thus sui table for use in pharmacokinetic studies of acyclovir. The method had a mean absolute recovery of 96%, while the within-day and between-day coefficients of variation and percentages error were all less than 8%. The calibration curve was linear over a concentration range of 62.5-4 000 ng/ml.