Js. Jeong et al., Changes in expression of cell cycle regulators and their hepatic lobular distribution in partial hepatectomy-induced regenerating rat liver, J KOR MED S, 14(6), 1999, pp. 635-642
Partial hepatectomy (PH) endorses quiescent hepatocytes to reenter the cell
cycle. The regenerating liver returns to its preresection weight after 7 d
ays, following one or two cell division and maintains nearly its original v
olume after then. We focused on the inhibition of further hepatocyte prolif
eration, hypothesizing possible involvement of cell cycle upregulators and
inhibitors, We studied protein levels in expression of cyclins, cyclin depe
ndent kinases (CDKs) and CDK inhibitors (CKIs), and their in situ hepatic l
obular distributions in partial hepatectomized rat liver. Cyclin E was expr
essed in the same levels in normal liver and after PH, Expression of cyclin
A, not detected in normal liver, increased in following times after PH and
reached a maximum at 7 day. CDK2 and 4 showed increased expression toward
terminal period. Contradictory findings of cyclin A and these CDKs might pl
ay an important role in the inhibition of further cell division, although s
till unclear. Constitutively expressed CDK6 decreased after 1 day, p18 show
ed peak expression within 1 day, and p16, p21, p27 and p57 were stronger at
terminal periods. During the expected period of their activity, intranucle
ar translocations were observed in cyclin E, p18 and p16. There was no evid
ence of regional distribution in hepatic lobular architecture, instead, dif
fuse in situ expression, corroborating synchronous event, was found.