Animation of the dynamical events of the elongation cycle based on cryoelectron microscopy of functional complexes of the ribosome

Using three-dimensional cryoelectron microscopy, the binding positions of t RNA and elongation factors EF-Gr and EF-Tu (the latter complexed with amino acyl tRNA and GTP) on the ribosome were determined in previous studies, On the basis of these studies, the dynamical events that take place in the cou rse of the elongation cycle of protein synthesis have been animated. The re sulting 3-min movie is accessible on the website of this journal (http://ww w.idealibrary.com). The following article provides a brief annotation of th ose frames of the movie for which experimental support is available. (C) 19 99 Academic Press.