Concentration of carotenoid in satsuma mandarin (Citrus unshiu marc.) pulpby freezing and thawing treatment

We prepared carotenoid rich pulp by centrifuging Satsuma mandarin juice twi ce. The freezing and thawing treatment was examined in order to increase ca rotenoid quantity included in the pulp. After the freezing and thawing trea tment was applied to the pulp, a centrifugal separation was carried out. Ho wever, the effect which raised the carotenoid quantity of the pulp was low in this treatment because of a lot of soluble solid included in the pulp. T hen the freezing and thawing treatment was carried out against the pulp wit h an addition of 0.1% pectinase. The carotenoid quantity in the sediment co uld be increased, when the treated pulp was centrifuged. It is considered t hat this increase results from the cooperative effect of the enzyme reactio n at low temperatures and aggregating the pulp by the freezing and thawing treatment. The freezing and thawing treatment combined with pectinase treat ment is practical because it is easy to operate in a large scale manufactur e. Also, we can easily manage this process in or out of the Satsuma mandari n juice extraction season.