Establishment of a myelodysplastic syndrome (MDS)/secondary AML-derived T lymphoid cell line K2-MDS

We have established a T lymphoid cell line, K2-MDS, from the peripheral blo od mononuclear cells (PBMC) of a patient with acute myeloblastic leukemia ( AML) transformed myelodysplastic syndrome (MDS). K2-MDS cells are positive for the expression of CD4, CD5, CD13, CD25, CD71, CD95, HLA-DR and cytoplas mic CD3. Southern blotting analysis shows T cell receptor (TCR) beta chain genes rearrangements, whereas immunoglobulin heavy chain (IgH) genes are no t rearranged. Further, the patient PBMC contains TCR beta chain genes rearr angements in the same manner as K2-MDS cells. The data indicate that K2-MDS is a T lymphoid cell line derived from a myelodysplastic clone in the pati ent PBMC. This new MDS-derived cell line K2-MDS may be a useful in vitro mo del for studies on the pathogenetic mechanisms leading to MDS. (C) 2000 Els evier Science Ltd. All rights reserved.