Lack of interaction in recombinant human FSH receptor and both TSAb and TSBAb

Since cross-reactivity of TSH with the human FSH receptor has been reported , in this study we tested the effect of thyroid-stimulating antibody (TSAb) and thyroid stimulation-blocking antibody (TSBAb) on Chinese hamster ovary cells expressing human FSH receptor (CHO-hFSH-R cells). We examined the TS BAb, activity of sera from hypothyroid patients who had a positive TBII to determine whether these sera also block the effect of FSH on CHO-hFSH-R cel ls. Although human FSH I-3 ( 0.25-16 ng/ml) stimulated the production of in tracellular cAMP in CHO-hFSH-R cells with dose-responsive manner, neither T SAb nor TSBAb had such an effect on the cells.