S. Sukowaki et al., Isozyme evidence for three sibling species in the Anopheles sundaicus complex from Indonesia, MED VET ENT, 13(4), 1999, pp. 408-414
Electrophoretic studies of isoenzymes in three chromosomally distinct forms
(A, B and C) of the mosquito Anopheles sundaicus Theobald (Diptera: Culici
ae) were undertaken on wild samples collected from six geographically isola
ted populations in Indonesia. Analyses of 12 enzyme systems comprising 15 l
oci revealed significant allelic variations, genetic polymorphism, within a
nd among the populations of the An. sundaicus complex. Phylogenetic dendrog
rams produced by analysis using the Biosys-1 program based on UPGMA methods
show that all the populations of form A fall into one cluster, which is cl
osely related to the form C cluster, whereas the populations of form B belo
ng to a more distinct cluster. Allelic frequency and Wright's F statistics
of Mpi (mannose phosphate isomerase) are sufficient to identify individuals
of each cytological form. This isozyme data correlates with our previous c
ytological evidence for the existence of three isomorphic species within th
e taxon An. sundaicus in Indonesia. These three species of the An. sundaicu
s complex were found together sympatrically at one locality, Asahan in Nort
h Sumatra.