Functional analysis of 150 deletion mutants in Saccharomyces cerevisiae bya systematic approach

In a systematic approach to the study of Saccharomyces cerevisiae genes of unknown function, 150 deletion mutants were constructed (1 double, 149 sing le mutants) and phenotypically analysed. Twenty percent of all genes examin ed were essential. The viable deletion mutants were subjected to 20 differe nt test systems, ranging from high throughput to highly specific test syste ms. Phenotypes were obtained for two-thirds of the mutants tested. During t he course of this investigation, mutants for 26 of the genes were described by others. For is of these the reported data were in accordance with our r esults. Surprisingly, for seven genes, additional, unexpected phenotypes we re found in our tests. This suggests that the type of analysis presented he re provides a more complete description of gene function.