Bp. Eliceiri et al., Selective requirement for Src kinases during VEGF-induced angiogenesis andvascular permeability, MOL CELL, 4(6), 1999, pp. 915-924
Src kinase activity was found to protect endothelial cells from apoptosis d
uring vascular endothelial growth factor (VEGF)-, but not basic fibroblast
growth factor (bFGF)-, mediated angiogenesis in chick embryos and mice. In
fact, retroviral targeting of kinase-deleted Src to tumor-associated blood
vessels suppressed angiogenesis and the growth of a VEGF-producing tumor. A
lthough mice lacking individual Src family kinases (SFKs) showed normal ang
iogenesis, mice deficient in pp60(c-src) or pp62(c-yes) showed no VEGF-indu
ced vascular permeability (VP), yet fyn(-/-) mice displayed normal VP. In c
ontrast, inflammation-mediated VP appeared normal in Src-deficient mice. Th
erefore, VEGF-, but not bFGF-, mediated angiogenesis requires SFK activity
in general, whereas the VP activity of VEGF specifically depends on the SFK
s, Src, or Yes.