Kc. Jung et al., Development of new adherent mutant from human myeloma-derived cell line: In vitro model of anaplastic transformation of myeloma, MOL CELLS, 9(6), 1999, pp. 657-661
Anaplastic myeloma is a rare but distinct, biologically aggressive variant
of myeloma which usually results from dedifferentiation or anaplastic trans
formation of the myeloma cells. The molecular mechanisms that determine the
biologic behavior of anaplastic myeloma and effective treatment modalities
have not been well known due to lack of in vitro models. In the present st
udy, we have developed an anaplastically transformed mutant from a human my
eloma-derived cell line. In the process of long-term culture of the myeloma
-derived IM-9 cell line in low serum and nutrient conditions, an adherent m
utant line was developed and named IM-9/AD. This mutant cell line displayed
several characteristics resembling anaplastic myeloma such as: 1, large ce
lls with large vesicular nucleus and prominent nucleolus, multinuclearity a
nd high mitotic figures; 2, loss of leukocyte-associated antigens; and 3, h
igher tumorigenecity in scid mice than its parental cell line. This newly d
eveloped mutant cell line may serve as a readily available in vitro model t
o investigate the biology of anaplastic myeloma.