I. Gorman et al., EFFECT OF TEMPERATURE AND DIETARY-SODIUM BICARBONATE SUPPLEMENTATION ON THE MINERAL EXCRETION OF BROILERS, Australian Journal of Agricultural Research, 48(5), 1997, pp. 703-707
A non-surgical technique was developed for the collection of urine sam
ples free from faecal contamination. This technique was used with grow
ing broilers to study the effects of ambient temperature and dietary s
odium bicarbonate (NaHCO3) supplementation on the urinary excretion of
mineral ions 1.5-2.0 h after feeding. High ambient temperatures resul
ted in increases in the absolute retentions of sodium, potassium, calc
ium, magnesium, phosphorus, and sulfur. The lack of any temperature ef
fect on the urinary excretion of the non-monovalent ions indicated tha
t the changes in retentions of these ions were due to changes in absor
ption. The increase in potassium retention was related to a decrease i
n urinary excretion, as would be expected for an ion regulated predomi
nantly by the kidneys. However, no effect of temperature was observed
on the urinary excretion of sodium. Sodium bicarbonate supplementation
resulted in an increase in both the absolute retention and urinary ex
cretion of sodium. Other minerals were unaffected by NaHCO3 supplement
ation other than for a marginally significant (P = 0.05) increase in t
he absolute retention of sulfur. In order to minimise faecal contamina
tion of the urine samples, birds were fasted for 16 h prior to feeding
with experimental diets. Possible problems with tissue catabolism wer
e minimised by supplying birds with a glucose-casein solution during t
he fasting period.