Chlorogenic acid in a Nicotiana plumbaginifolia cell suspension

A phenylpropanoid compound has been characterized in a Nicotiana plumbagini folia cell suspension. This compound has been isolated and purified by semi -preparative reverse phase-high performance liquid chromatography. Its stru cture has been identified by NMR spectroscopy as 5-O-caffeoylquinic acid, w hich is chlorogenic acid (CA). The influence of culture conditions on the a ccumulation of this metabolite by N. plumbaginifolia cell suspensions has b een studied. Darkness strongly inhibits the CA accumulation. Moreover, it h as been shown that feeding experiments with caffeic acid had a deleterious effect upon the CA content. This one was not influenced by a supplementatio n with quinic acid. (C) 1999 Editions scientifiques et medicales Elsevier S AS.