Determination of the compositional distribution of copolymers by frontal analysis continuous capillary electrophoresis

Frontal analysis continuous capillary electrophoresis (FACCE) was carried o ut for a series of random copolymers of an ionic monomer, sodium 2-(acrylam ido)-2-methylpropanesulfonate (AMPS), and a nonionic monomer, acrylamide (A AM). The electropherograms appeared in order of anionic content and were ge nerally sigmoidal, in contrast to that of hyaluronic acid (HA), which was a brupt and discontinuous. This difference could be related to the compositio nal heterogeneity of the copolymers, completely absent in the biopolymer, T hrough the range of copolymer composition (10-100% AMPS) the relationship b etween average mobility and nominal AMPS content could be described by a ca libration curve, making it possible to deduce the compositional distributio n of copolymer samples.