Safety evaluation of sons vide-processed products with respect to nonproteolytic Clostridium botulinum by use of challenge studies and predictive microbiological models
E. Hyytia-trees et al., Safety evaluation of sons vide-processed products with respect to nonproteolytic Clostridium botulinum by use of challenge studies and predictive microbiological models, APPL ENVIR, 66(1), 2000, pp. 223-229
Sixteen different types of sous vide-processed products were evaluated for
safety with respect to nonproteolytic group IT Clostridium botulinum by usi
ng challenge tests with low (2.0-log-CFU/kg) and high (5.3-log-CFU/kg) inoc
ula and two currently available predictive microbiological models, Food Mic
roModel (FMM) and Pathogen Modeling Program (PMP). After thermal processing
, the products were stored at 4 and 8 degrees C and examined for the presen
ce of botulinal spores and neurotoxin on the sell-by date and 7 days after
the sell-by date. Most of the thermal processes were found to be inadequate
for eliminating spores, even in low-inoculum samples. Only 2 of the 16 pro
ducts were found to be negative for botulinal spores and neurotoxin at both
sampling times, Two products at the high inoculum level showed toxigenesis
during storage at 8 degrees C, one of them at the sell-by date. The predic
tions generated by both the FMM thermal death model and the FMM and PMP gro
wth models were found to be inconsistent with the observed results in a maj
ority of the challenges. The inaccurate predictions were caused by the limi
ted number and range of the controlling factors in the models. Based on thi
s study, it was concluded that the safety of sous vide products needs to be
carefully evaluated product by product, Time-temperature combinations used
in thermal treatments should be reevaluated to increase the efficiency of
processing, and the use of additional antibotulinal hurdles, such as biopre
servatives, should be assessed.