Specific detection of Leuconostoc mesenteroides subsp mesenteroides with DNA primers identified by randomly amplified polymorphic DNA analysis

Authors
Moschetti, G Blaiotta, G Villani, F Coppola, S
Citation
G. Moschetti et al., Specific detection of Leuconostoc mesenteroides subsp mesenteroides with DNA primers identified by randomly amplified polymorphic DNA analysis, APPL ENVIR, 66(1), 2000, pp. 422-424
Citations number
11
Categorie Soggetti
Biology,Microbiology
Journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN journal
00992240 → ACNP
Volume
66
Issue
1
Year of publication
2000
Pages
422 - 424
Database
ISI
SICI code
0099-2240(200001)66:1<422:SDOLMS>2.0.ZU;2-3
Abstract
Randomly amplified polymorphic DNA analysis using primer 239 (5' CTGAAGCGGA 3') was performed to characterize Leuconostoc sp. strains. All the strains of leuconostoc mesenteroides subsp. mesenteroides (with the exception of t wo strains), two strains formerly identified as L. gelidum, and one strain of Leuconostoc showed a common band at about 1.1 kb. This DNA fragment was cloned and sequenced in order to verify its suitability for identifying L. mesenteroides subsp. mesenteroides strains.