J. Tada et al., Involvement of vesicle-cytoskeleton interaction in AQP5 trafficking in AQP5-gene-transfected HSG cells, BIOC BIOP R, 266(2), 1999, pp. 443-447
Citations number
22
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
A cDNA of rat aquaporin 5 (AQP5) was used to transfect to HSG (human saliva
ry gland cells), and the trafficking mechanism was studied in vitro by conf
ocal laser microscopy. The trafficking of AQP5 to the plasma membrane was i
nduced by stimulation of AQP5-gene-transfected human salivary gland cells (
HSGAQP5 cells) with thapsigargin, an inhibitor of endoplasmic Ca2+-ATPase,
and or with A-23187, a calcium ionophore, Pretreatment of these cells with
colchicine or vinblastine, microtubule inhibitors, prevented the traffickin
g induced by thapsigargin or A-23187. The trafficking event was not complet
ely inhibited by cytochalasin B, a microfilament inhibitor. These results d
emonstrate that the trafficking of AQP5 vesicles to the plasma membrane is
triggered by an increase in intracellular Ca2+ and that the interaction of
AQP5-containing vesicles with the cytoskeleton is involved in this traffick
ing. (C) 1999 Academic Press.